BEIRUT: A Syrian rescue service operating in rebel-held territory said on Tuesday that a helicopter dropped containers of toxic gas overnight on a town close to where a Russian military helicopter was shot down hours earlier, Reuters news service reported. The opposition Syrian National Coalition (SNC) accused Syrian President Bashar al Assad of being behind the attack. Assad has denied the previous accusations of using chemical weapons. A spokesman for the Syria Civil Defense said that 33 people, mostly women and children, were affected by the gas, which they suspect was chlorine, in Saraqeb in the rebel-held Idlib province. The group, which describes itself as a neutral band of search and rescue volunteers, posted a video on YouTube apparently showing a number of men struggling to breathe and being given oxygen masks by people in civil defense uniforms. The SNC said in a statement, “After shelling, besieging and killing civilians and perpetrating war crimes on them, the Assad regime has resorted once again, and in breach of UN resolutions 2118 and 2235, to using chemical substances and toxic gasses.” It added that the daily reality confirms that all the international agreements and previous UN Security Council decisions, about the chemical weapons or otherwise, were meaningless for the Assad regime. The Civil Defence spokesman said that it was the second time Saraqeb had been hit by a toxic gas. The group was aware of around nine suspected chlorine gas incidents across Idlib province since the conflict began, he said. Monitors at the Britain-based Syrian Observatory for Human Rights, which tracks violence on all sides in the civil war, said that barrel bombs fell on Saraqeb late on Monday, wounding a large number of citizens. Russia’s defense ministry said that a Russian helicopter was shot down near Saraqeb during the day on Monday, killing all five people on board, in the biggest officially acknowledged loss of life for Russian forces since they started operations in Syria. The helicopter came down roughly mid-way between Aleppo and Russia’s main air base at Khmeimim in the western province of Latakia, near the Mediterranean coast. Russian air power began supporting Assad late last year. This was an intervention which tipped the balance of the war in Assad’s favor, eroding gains the rebels had made that year. The Russian defense ministry said that the Mi-8 military transport helicopter was shot down after delivering humanitarian aid to Aleppo as it made its way back to Khmeimim. No group has claimed responsibility for downing the helicopter. The government and opposition forces have both denied using chemical weapons during the five-year-old civil war. Western powers said that the government had been responsible for chlorine and other chemical attacks. The government and Russia have accused rebels of using poison gas. The UN investigators established that sarin gas was used in Eastern Ghouta in 2013. The United States accused Damascus of that attack, which it estimates killed 1,429 people, including at least 426 children. Damascus denied responsibility, and blamed rebels. Later that year the UN and the Syrian government agreed to destroy the state’s declared stockpile of chemical weapons, a process completed in January 2016. The Organisation for the Prohibition of Chemical Weapons confirmed in late 2015 that sulfur mustard, commonly known as mustard gas, had been used for the first time in the conflict, without saying which party in the many sided conflict it thought had used it.