The National Institute of Health (NIH) in collaboration with Fleming Fund Country Grant to Pakistan conducted a two-day National Symposium on Antimicrobial Resistance (AMR). Due to the ongoing pandemic, the symposium was semi-virtual and centrally located in Islamabad. It provided a platform to understand the challenges, impact and progress of AMR in human, animal and environmental health sectors across Pakistan. AMR is one of the biggest public health threats in the world today. It represents the capability of microorganisms to evolve in such a manner as to render the antimicrobials we have long used against them as ineffective.While chairing the national virtual symposium, Executive Director NIH Maj Gen Prof Aamer Ikram said, “It is a fact, not everyone is aware that antibiotics need to be used in an appropriate manner to prevent the emergence of the resistant superbugs. This kind of a symposium has provided a much-needed platform for the key stakeholders to sit together and introduce massive changes to the present practices of the use of antimicrobial agents in human, animal and environmental health sectors under a collective One Health approach.” As a commitment to the World Health Assembly Resolution 2015 (WHA68.7), Pakistan had developed its National AMR Framework, National Action Plan (NAP) and a multisectoral National AMR Steering Control Committee in 2017. Keeping in view the importance of the issue, the Government of Pakistan has approved funding to launch National Program on AMR and IPC based at NIH.The symposium provided all the key stakeholders an opportunity for further proceeding. The representatives from MoNHSR&C, MoHFS&R, MoCC, provincial health and livestock departments, academia, international organizations, private organizations, hospitals, clinicians, politicians and bureaucrats attended the Symposium. “The Government of Pakistan is committed to play its part in the global disease control programs more effectively and stand alongside the global community in their effort to reduce the burden of AMR.” Dr. Nausheen Hamid, Parliamentary Secretary for Health, added. “We are grateful to the international community as well as for our technical partners.”The UK established Fleming Fund supports the Government of Pakistan to implement the AMR strategy within the country. This grant for AMR is intended to develop and implement a thorough plan on AMR surveillance capacity building involving the human and animal health sectors and other related stakeholders. The National Action Plan lists AMR surveillance as one of seven priority areas. It includes upgradation of the public and animal health laboratories, capacity building of the laboratory staff working on AMR, development and implementation of AMR surveillance plans in both human and animal health sectors, and survey collection for development of AMC and AMU data baseline.