Environmental surveillance has detected wild poliovirus in sewage samples from 20 districts across Pakistan, officials said, though data suggests a decline in overall virus circulation. According to the Regional Reference Laboratory for Polio Eradication at the National Institute of Health (NIH), 60 samples were collected from 51 districts. Of these, 25 tested positive while 35 showed no presence of the virus. The samples that confirmed the presence of wild poliovirus type 1 (WPV1) came from districts including Dukki, Kech, Khuzdar, Lasbela, Loralai, Nasirabad, Pishin, Quetta, Usta Muhammad, Bannu, Kohat, Lakki Marwat, Peshawar, South Waziristan Lower, Bahawalpur, Bahawalnagar, Dera Ghazi Khan, Lahore, Multan, and Rahim Yar Khan. Meanwhile, samples from cities such as Islamabad, Gilgit, Rawalpindi, Swat, Sahiwal, Nowshera, and others tested negative, pointing to improved control measures in several areas.